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In brief, EIA microplates (Corning CLS3590) were coated overnight with 100 µl of coating buffer (carbonate-bicarbonate with pH = 9.6, Sigma C3041) or phage Muddy diluted to 5 ×10 9 pfu ml −1 in coating buffer

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Tests for GHD include measurement of the hormone in urine or serum or measurement of stimulated GH levels after administration of various provocative agents [16]